Tpm1 Knockout cell line (H9C2)

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location： Home > Products > KO Cell Lines > Tpm1 Knockout cell line (H9C2)

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Tpm1 Knockout cell line (H9C2) Order

Tpm1 Knockout cell line (H9C2)
Catalog#
YKO-R047
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Tpm1 Knockout cell line (H9C2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Tpm1 Knockout cell line (H9c2(2-1))
Morphology
myoblast,adherent
Passage ratio
1:3~1:4
Species
Rat
Gene Name
Tpm1
Gene ID
24851
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Target region
Knockout region: Exon 4 (based on transcript Tpm1-206)
Validation: PCR+Sanger Sequencing
Tpm1 Knockout cell line (H9C2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Tpm1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9c2(2-1) cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Tpm1 gene knockout Tpm1 Knockout cell line (H9c2(2-1)) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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